Biology:5β-Reductase
| Δ4-3-Oxosteroid 5β-reductase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 1.3.1.3 | ||||||||
| CAS number | 2604034 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
5β-Reductase, or Δ4-3-oxosteroid 5β-reductase (EC 1.3.1.3, 3-oxo-Δ4-steroid 5β-reductase, androstenedione 5β-reductase, cholestenone 5β-reductase, cortisone 5β-reductase, cortisone Δ4-5β-reductase, steroid 5β-reductase, testosterone 5β-reductase, Δ4-3-ketosteroid 5β-reductase, Δ4-5β-reductase, Δ4-hydrogenase, 4,5β-dihydrocortisone:NADP+ Δ4-oxidoreductase, 3-oxo-5β-steroid:NADP+ Δ4-oxidoreductase) is an enzyme with systematic name 5β-cholestan-3-one:NADP+ 4,5-oxidoreductase.[1][2][3][4][5][6][7][8][9] This enzyme catalyses the following chemical reaction
- (1) 5β-cholestan-3-one + NADP+ [math]\displaystyle{ \rightleftharpoons }[/math] cholest-4-en-3-one + NADPH + H+
- (2) 17,21-dihydroxy-5β-pregnane-3,11,20-trione + NADP+ [math]\displaystyle{ \rightleftharpoons }[/math] cortisone + NADPH + H+
The human enzyme efficiently acts on progesterone, 17α-hydroxyprogesterone, androstenedione, and testosterone to 5β-reduced metabolites. It can also act on aldosterone, corticosterone and cortisol, to some extent.
See also
References
- ↑ "Separation of delta 4-5 alpha-hydrogenases from rat liver homogenates". The Journal of Biological Chemistry 223 (1): 443–8. November 1956. PMID 13376613.
- ↑ "The delta4-hydrogenases of guinea pig adrenal gland". The Journal of Biological Chemistry 235: 1317–20. May 1960. PMID 13805063.
- ↑ Levy, H.R.; Talalay, P. (1957). "Enzymatic introduction of double bonds into steroid ring A". J. Am. Chem. Soc. 79 (10): 2658–2659. doi:10.1021/ja01567a089.
- ↑ "The enzymatic reduction of delta 4-3-ketosteroids". The Journal of Biological Chemistry 225 (1): 13–24. March 1957. PMID 13416214.
- ↑ "Purification of 5 beta-reductase from hepatic cytosol fraction of chicken". The Journal of Steroid Biochemistry and Molecular Biology 37 (5): 717–24. December 1990. doi:10.1016/0960-0760(90)90356-p. PMID 2278855.
- ↑ "Identification of cortisone 5 beta-reductase as delta 4-3-ketosteroid 5 beta-reductase". Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology 912 (1): 110–4. March 1987. doi:10.1016/0167-4838(87)90253-6. PMID 3828348.
- ↑ "Purification and characterization of delta 4-3-ketosteroid 5 beta-reductase". The Journal of Biological Chemistry 259 (12): 7519–24. June 1984. PMID 6736016.
- ↑ "Genomic organization of a human 5beta-reductase and its pseudogene and substrate selectivity of the expressed enzyme". Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression 1517 (2): 228–35. January 2001. doi:10.1016/s0167-4781(00)00278-5. PMID 11342103.
- ↑ "Cloning and expression of cDNA of human delta 4-3-oxosteroid 5 beta-reductase and substrate specificity of the expressed enzyme". European Journal of Biochemistry 219 (1–2): 357–63. January 1994. doi:10.1111/j.1432-1033.1994.tb19947.x. PMID 7508385.
External links
- Delta4-3-oxosteroid+5beta-reductase at the US National Library of Medicine Medical Subject Headings (MeSH)
 |  |
