Biology:N-acetylneuraminate synthase

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Short description: Class of enzymes
N-acetylneuraminate synthase
1xuz.jpg
N-acetylneuraminic acid synthase dimer, Neisseria meningitidis
Identifiers
EC number2.5.1.56
CAS number37290-66-7
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO

In enzymology, a N-acetylneuraminate synthase (EC 2.5.1.56) is an enzyme that catalyzes the chemical reaction

phosphoenolpyruvate + N-acetyl-D-mannosamine + H2O [math]\displaystyle{ \rightleftharpoons }[/math] phosphate + N-acetylneuraminate

The 3 substrates of this enzyme are phosphoenolpyruvate, N-acetyl-D-mannosamine, and H2O, whereas its two products are phosphate and N-acetylneuraminate.

This enzyme belongs to the family of transferases, specifically those transferring aryl or alkyl groups other than methyl groups. The systematic name of this enzyme class is phosphoenolpyruvate:N-acetyl-D-mannosamine C-(1-carboxyvinyl)transferase (phosphate-hydrolysing, 2-carboxy-2-oxoethyl-forming). Other names in common use include (NANA)condensing enzyme, N-acetylneuraminate pyruvate-lyase (pyruvate-phosphorylating), and NeuAc synthase. This enzyme participates in aminosugars metabolism.

Structural studies

As of late 2007, only one structure has been solved for this class of enzymes, with the PDB accession code 1WVO.

References

  • "Biosynthesis of sialic acids by Neisseria meningitidis". J. Biol. Chem. 237: 3520–6. 1962. PMID 13971393. 
  • "Purification and characterization of N-acetylneuraminate synthase from Escherichia coli K1-M12". Biosci. Biotechnol. Biochem. 61 (12): 2046–50. 1997. doi:10.1271/bbb.61.2046. PMID 9438985.