Biology:Methylaspartate ammonia-lyase

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methylaspartate ammonia-lyase
1kko.png
X-ray structure of methylaspartate ammonia lyase. PDB entry 1kko[1]
Identifiers
EC number4.3.1.2
CAS number9033-26-5
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO

The enzyme methylaspartate ammonia-lyase (EC 4.3.1.2) catalyzes the chemical reaction

L-threo-3-methylaspartate [math]\displaystyle{ \rightleftharpoons }[/math] mesaconate + NH3

This enzyme belongs to the family of lyases, specifically ammonia lyases, which cleave carbon-nitrogen bonds. The systematic name of this enzyme class is L-threo-3-methylaspartate ammonia-lyase (mesaconate-forming). Other names in common use include β-methylaspartase, 3-methylaspartase, and L-threo-3-methylaspartate ammonia-lyase. This enzyme participates in c5-branched dibasic acid metabolism and nitrogen metabolism. It employs one cofactor, cobamide.

Structural studies

Several structures of this enzyme have been deposited in the Protein Data Bank (linked in the infobox) which show it possesses a TIM barrel domain.

References

  1. Levy, C. W.; Buckley, P. A.; Sedelnikova, S.; Kato, Y.; Asano, Y.; Rice, D. W.; Baker, P. J. (2002). "Insights into Enzyme Evolution Revealed by the Structure of Methylaspartate Ammonia Lyase". Structure 10 (1): 105–13. doi:10.1016/S0969-2126(01)00696-7. PMID 11796115.