Biology:Eukaryotic translation termination factor 1

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Short description: Protein-coding gene in the species Homo sapiens


A representation of the 3D structure of the protein myoglobin showing turquoise α-helices.
Generic protein structure example

Eukaryotic translation termination factor 1 (eRF1), also known as TB3-1, is a protein that in humans is encoded by the ETF1 gene.[1][2][3]

In eukaryotes and archaea, this is the sole class 1 release factor (eRF) which recognizes all three stop codons. The overall process of termination is similar in bacteria, but in the latter 2 separate codon-recognizing release factors exist, RF1 and RF2. [4]

Function

Termination of protein biosynthesis and release of the nascent polypeptide chain are signaled by the presence of an in-frame stop codon at the aminoacyl site of the ribosome. The process of translation termination is universal and is mediated by protein release factors (RFs) and GTP. A class 1 RF recognizes the stop codon and promotes the hydrolysis of the ester bond linking the polypeptide chain with the peptidyl site tRNA, a reaction catalyzed at the peptidyl transferase center of the ribosome. Class 2 RFs, which are not codon specific and do not recognize codons, stimulate class 1 RF activity and confer GTP dependency upon the process. In bacteria, both class 1 RFs, RF1 and RF2, recognize UAA; however, UAG and UGA are decoded specifically by RF1 and RF2, respectively. In eukaryotes, eRF1, or ETF1, the functional counterpart of RF1 and RF2, functions as an omnipotent RF, decoding all 3 stop codons.[1][3]

References

  1. 1.0 1.1 "Entrez Gene: eukaryotic translation termination factor 1". https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=2107. 
  2. "Chromosomal localization of a human cDNA containing a DIDS binding domain and demonstrating high homology to yeast omnipotent suppressor 45". Somat. Cell Mol. Genet. 18 (1): 97–102. January 1992. doi:10.1007/BF01233452. PMID 1546371. 
  3. 3.0 3.1 "A highly conserved eukaryotic protein family possessing properties of polypeptide chain release factor". Nature 372 (6507): 701–3. December 1994. doi:10.1038/372701a0. PMID 7990965. Bibcode1994Natur.372..701F. 
  4. "Termination of translation: interplay of mRNA, rRNAs and release factors?". EMBO J. 22 (2): 175–82. January 2003. doi:10.1093/emboj/cdg017. PMID 12514123. 

Further reading

External links

This article incorporates text from the United States National Library of Medicine, which is in the public domain.