Biology:Cyanophycin synthase (L-arginine-adding)

From HandWiki
Short description: Class of enzymes
Cyanophycin synthase (L-arginine-adding)
Identifiers
EC number6.3.2.30
CAS number131554-17-1
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum

Cyanophycin synthase (L-arginine-adding) (EC 6.3.2.30, CphA, CphA1, CphA2, cyanophycin synthetase, multi-L-arginyl-poly-L-aspartate synthase) is an enzyme with systematic name cyanophycin:L-arginine ligase (ADP-forming).[1][2][3][4][5][6] This enzyme catalyses the following chemical reaction:

ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg [math]\displaystyle{ \rightleftharpoons }[/math] ADP + phosphate + [L-Asp(4-L-Arg)]n + 1

This enzyme requires Mg2+ for activity. This enzyme requires Mg2+ for activity. All enzymes known to have this activity also catalyze the addition of aspartate, i.e. cyanophycin synthase (L-aspartate-adding) activity. It is structurally similar to Muramyl ligases.

References

  1. "Molecular characterization of the cyanophycin synthetase from Synechocystis sp. strain PCC6308". Archives of Microbiology 174 (5): 297–306. November 2000. doi:10.1007/s002030000206. PMID 11131019. 
  2. "Purification of Synechocystis sp. strain PCC6308 cyanophycin synthetase and its characterization with respect to substrate and primer specificity". Applied and Environmental Microbiology 67 (5): 2176–82. May 2001. doi:10.1128/AEM.67.5.2176-2182.2001. PMID 11319097. 
  3. "Cyanophycin granule polypeptide formation and degradation in the cyanobacterium Aphanocapsa 6308". Journal of Bacteriology 141 (2): 687–93. February 1980. PMID 6767688. 
  4. "Biosynthesis of the cyanobacterial reserve polymer multi-L-arginyl-poly-L-aspartic acid (cyanophycin): mechanism of the cyanophycin synthetase reaction studied with synthetic primers". European Journal of Biochemistry 267 (17): 5561–70. September 2000. doi:10.1046/j.1432-1327.2000.01622.x. PMID 10951215. 
  5. "Cyanophycin synthetase-like enzymes of non-cyanobacterial eubacteria: characterization of the polymer produced by a recombinant synthetase of Desulfitobacterium hafniense". Zeitschrift für Naturforschung C 57 (5-6): 522–9. 2002. doi:10.1515/znc-2002-5-621. PMID 12132696. 
  6. "Molecular characterization of cyanophycin synthetase, the enzyme catalyzing the biosynthesis of the cyanobacterial reserve material multi-L-arginyl-poly-L-aspartate (cyanophycin)". European Journal of Biochemistry 254 (1): 154–9. May 1998. doi:10.1046/j.1432-1327.1998.2540154.x. PMID 9652408. 

External links