Biology:Clavaminate synthase
From HandWiki
| Clavaminate synthase | |||||||||
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| Identifiers | |||||||||
| EC number | 1.14.11.21 | ||||||||
| CAS number | 122799-56-8 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
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Clavaminate synthase (EC 1.14.11.21, clavaminate synthase 2, clavaminic acid synthase) is an enzyme with systematic name deoxyamidinoproclavaminate,2-oxoglutarate:oxygen oxidoreductase (3-hydroxylating).[1][2][3][4][5] This enzyme catalyses the following chemical reaction
- (1) deoxyamidinoproclavaminate + 2-oxoglutarate + O2 [math]\displaystyle{ \rightleftharpoons }[/math] amidinoproclavaminate + succinate + CO2
- (2) proclavaminate + 2-oxoglutarate + O2 [math]\displaystyle{ \rightleftharpoons }[/math] dihydroclavaminate + succinate + CO2 + H2O
- (3) dihydroclavaminate + 2-oxoglutarate + O2 [math]\displaystyle{ \rightleftharpoons }[/math] clavaminate + succinate + CO2 + H2O
Clavaminate synthase contains nonheme iron.
References
- ↑ "Elucidation of the order of oxidations and identification of an intermediate in the multistep clavaminate synthase reaction". Biochemistry 30 (8): 2281–92. February 1991. doi:10.1021/bi00222a034. PMID 1998687.
- ↑ Zhou, J.; Gunsior, M.; Bachmann, B.O.; Townsend, C.A.; Solomon, E.I. (1998). "Substrate binding to the α-ketoglutarate-dependent non-heme iron enzyme clavaminate synthase 2: Coupling mechanism of oxidative decarboxylation and hydroxylation". J. Am. Chem. Soc. 120: 13539–13540. doi:10.1021/ja983534x.
- ↑ "Structural origins of the selectivity of the trifunctional oxygenase clavaminic acid synthase". Nature Structural Biology 7 (2): 127–33. February 2000. doi:10.1038/72398. PMID 10655615.
- ↑ "Spectroscopic studies of substrate interactions with clavaminate synthase 2, a multifunctional alpha-KG-dependent non-heme iron enzyme: correlation with mechanisms and reactivities". Journal of the American Chemical Society 123 (30): 7388–98. August 2001. doi:10.1021/ja004025+. PMID 11472170.
- ↑ "New reactions in clavulanic acid biosynthesis". Current Opinion in Chemical Biology 6 (5): 583–9. October 2002. doi:10.1016/S1367-5931(02)00392-7. PMID 12413541.
External links
- Clavaminate+synthase at the US National Library of Medicine Medical Subject Headings (MeSH)
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