Biology:4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase

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Short description: Class of enzymes
4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase
Identifiers
EC number2.4.1.152
CAS number111310-38-4
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO

In enzymology, a 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase (EC 2.4.1.152) is an enzyme that catalyzes the chemical reaction

GDP-beta-L-fucose + 1,4-beta-D-galactosyl-N-acetyl-D-glucosaminyl-R [math]\displaystyle{ \rightleftharpoons }[/math] GDP + 1,4-beta-D-galactosyl-(alpha-1,3-L-fucosyl)-N-acetyl-D-glucosaminyl- R

Thus, the two substrates of this enzyme are GDP-beta-L-fucose and 1,4-beta-D-galactosyl-N-acetyl-D-glucosaminyl-R. Its 3 products are GDP, 1,4-beta-D-galactosyl-(alpha-1,3-L-fucosyl)-N-acetyl-D-glucosaminyl-, and R.

This enzyme belongs to the family of glycosyltransferases, specifically the hexosyltransferases. The systematic name of this enzyme class is GDP-beta-L-fucose:1,4-beta-D-galactosyl-N-acetyl-D-glucosaminyl-R 3-alpha-L-fucosyltransferase. Other names in common use include:

  • Lewis-negative alpha-3-fucosyltransferase
  • plasma alpha-3-fucosyltransferase
  • guanosine diphosphofucose-glucoside alpha1→3-fucosyltransferase
  • galactoside 3-fucosyltransferase
  • GDP-L-fucose:1,4-beta-D-galactosyl-N-acetyl-D-glucosaminyl-R
  • 3-L-fucosyltransferase
  • GDP-beta-L-fucose:1,4-beta-D-galactosyl-N-acetyl-D-glucosaminyl-R, and
  • 3-L-fucosyltransferase

This enzyme participates in 3 metabolic pathways: glycosphingolipid biosynthesis - neo-lactoseries, glycosphingolipid biosynthesis - globoseries, and glycan structures - biosynthesis 2.

Structural studies

As of late 2007, 3 structures have been solved for this class of enzymes, with PDB accession codes 2NZW, 2NZX, and 2NZY.

References

  • "Human salivary fucosyltransferases : evidence for two distinct alpha-3-L-fucosyltransferase activities one or which is associated with the Lewis blood group Le gene". Biochem. Biophys. Res. Commun. 100 (4): 1611–8. 1981. doi:10.1016/0006-291X(81)90703-8. PMID 7295318. 
  • "Glycosyltransferases involved in elongation of N-glycosidically linked oligosaccharides of the complex or N-acetyllactosamine type". Methods Enzymol.. Methods in Enzymology 98: 98–134. 1983. doi:10.1016/0076-6879(83)98143-0. ISBN 978-0-12-181998-9. PMID 6366476. 
  • "C-terminal amino acids of Helicobacter pylori alpha1,3/4 fucosyltransferases determine type I and type II transfer". J. Biol. Chem. 278 (24): 21893–900. 2003. doi:10.1074/jbc.M301704200. PMID 12676935.