Biology:2'-phosphotransferase
From HandWiki
Short description: Class of enzymes
| 2'-phosphotransferase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 2.7.1.160 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
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In enzymology, a 2'-phosphotransferase (EC 2.7.1.160) is an enzyme that catalyzes the chemical reaction
- 2'-phospho-[ligated tRNA] + NAD+ [math]\displaystyle{ \rightleftharpoons }[/math] mature tRNA + ADP-ribose 1,2-phosphate + nicotinamide + H2O
Thus, the two substrates of this enzyme are 2'-phospho-[ligated tRNA] and NAD+, whereas its 4 products are mature tRNA, ADP-ribose 1,2-phosphate, nicotinamide, and H2O.
This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor. The systematic name of this enzyme class is 2'-phospho-[ligated tRNA]:NAD+ phosphotransferase. Other names in common use include yeast 2'-phosphotransferase, Tpt1, Tpt1p, and 2'-phospho-tRNA:NAD+ phosphotransferase.
References
- "Substrate recognition by a yeast 2'-phosphotransferase involved in tRNA splicing and by its Escherichia coli homolog". Biochemistry 40 (46): 14098–105. 2001. doi:10.1021/bi011388t. PMID 11705403.
- "Transient ADP-ribosylation of a 2'-phosphate implicated in its removal from ligated tRNA during splicing in yeast". J. Biol. Chem. 274 (5): 2637–44. 1999. doi:10.1074/jbc.274.5.2637. PMID 9915792.
- "A 2'-phosphotransferase implicated in tRNA splicing is essential in Saccharomyces cerevisiae". J. Biol. Chem. 272 (20): 13203–10. 1997. doi:10.1074/jbc.272.20.13203. PMID 9148937.
- "An enzyme from Saccharomyces cerevisiae uses NAD+ to transfer the splice junction 2'-phosphate from ligated tRNA to an acceptor molecule". J. Biol. Chem. 266 (18): 11986–92. 1991. PMID 2050693.
- "A human homolog of the yeast gene encoding tRNA 2'-phosphotransferase: cloning, characterization and complementation analysis". Cell. Mol. Life Sci. 60 (8): 1725–32. 2003. doi:10.1007/s00018-003-3107-7. PMID 14504659.
- "Analysis of 2'-phosphotransferase (Tpt1p) from Saccharomyces cerevisiae: evidence for a conserved two-step reaction mechanism". RNA 11 (1): 99–106. 2005. doi:10.1261/rna.7194605. PMID 15611300.
- "Structure-function analysis of the yeast NAD+-dependent tRNA 2'-phosphotransferase Tpt1". RNA 11 (1): 107–13. 2005. doi:10.1261/rna.7193705. PMID 15611301.
- "Crystal structure of the RNA 2'-phosphotransferase from Aeropyrum pernix K1". J. Mol. Biol. 348 (2): 295–305. 2005. doi:10.1016/j.jmb.2005.02.049. PMID 15811369.
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