Biology:Sea anemone cytotoxic protein

From HandWiki
(Redirected from Chemistry:Actinotoxin)
Anemone_cytotox
PDB 1o72 EBI.jpg
crystal structure of the water-soluble state of the pore-forming cytolysin sticholysin ii complexed with phosphorylcholine
Identifiers
SymbolAnemone_cytotox
PfamPF06369
InterProIPR009104
SCOP21kd6 / SCOPe / SUPFAM
TCDB1.C.38
OPM superfamily168
OPM protein4tsy

In molecular biology, the sea anemone cytotoxic proteins are lethal pore-forming proteins, known collectively as actinoporins, a sub-class of cytolysins. There are several different groups of cytolysins based on their structure and function.[1] This entry represents the most numerous group, the 20kDa highly basic peptides. These cytolysins form cation-selective pores in sphingomyelin-containing membranes. Examples include equinatoxins (from Actinia equina), sticholysins (from Stichodactyla helianthus), magnificalysins (from Heteractis magnifica), and tenebrosins (from Actinia tenebrosa), which exhibit pore-forming, haemolytic, cytotoxic, and heart stimulatory activities.

Cytolysins adopt a stable soluble structure, which undergoes a conformational change when brought in contact with a membrane, leading to an active, membrane-bound form that inserts spontaneously into the membrane. They often oligomerise on the membrane surface, before puncturing the lipid bilayers, causing the cell to lyse. The 20kDa sea anemone cytolysins require a phosphocholine lipid headgroup for binding, however sphingomyelin is required for the toxin to promote membrane permeability.[2] The crystal structures of equinatoxin II [3] and sticholysin II [4] both revealed a compact beta-sandwich consisting of ten strands in two sheets flanked on each side by two short alpha-helices, which is a similar topology to osmotin. It is believed that the beta sandwich structure attaches to the membrane, while a three-turn alpha helix lying on the surface of the beta sheet may be involved in membrane pore formation, possibly by the penetration of the membrane by the helix.

References

  1. "Cytolytic peptide and protein toxins from sea anemones (Anthozoa: Actiniaria)". Toxicon 40 (2): 111–24. February 2002. doi:10.1016/S0041-0101(01)00191-X. PMID 11689232. 
  2. "Dissecting the actinoporin pore-forming mechanism". Structure 11 (11): 1312–3. November 2003. doi:10.1016/j.str.2003.10.007. PMID 14604518. 
  3. "Solution structure of the eukaryotic pore-forming cytolysin equinatoxin II: implications for pore formation". Journal of Molecular Biology 315 (5): 1219–29. February 2002. doi:10.1006/jmbi.2001.5321. PMID 11827489. 
  4. "Crystal and electron microscopy structures of sticholysin II actinoporin reveal insights into the mechanism of membrane pore formation". Structure 11 (11): 1319–28. November 2003. doi:10.1016/j.str.2003.09.019. PMID 14604522. 
This article incorporates text from the public domain Pfam and InterPro: IPR009104